Circular RNA (circRNA) can serve as a molecular sponge for microRNA (miRNA), forming the circRNA miRNA axis and regulating the expression of downstream proteins. Although this mechanism has been widely reported in various biological processes, there is still a lack of reliable and convenient methods to intuitively monitor and locate the interactions between circRNA and miRNA in living cells.
The research team led by Wang Gang from Shandong First Medical University designed multiple DNA probes and loaded them onto two-dimensional copper metal organic frameworks (2D Cu MOF) nanosheets for one-step analysis of circRNA miRNA axis. Nanoflakes can not only serve as powerful fluorescence quenchers, but also as excellent nanocarriers for abundant DNA probes for further assembly. Probes@Cu-MOF The complex can be used to track the circRNA miRNA axis in living cells, with high sensitivity and co localization analysis. This platform combines the transmembrane advantage of nanosheets with the signal amplification ability of local DNA cascade assembly, thus having great potential in understanding the biological functions of circRNA miRNA in cancer pathogenesis and therapeutic applications.
The study was published on March 11, 2023 in the international journal Biosensors and Bioelectronics, titled Tracing cellular interaction of circRNA miRNA axis with Cu metal organic framework supported DNA cascade assembly.
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Figure 1. The study was published in the international journal Biosensors and Bioelectronics
Figure 2. Images in the article
Figure 3. Cell culture in this study was conducted using Opsey fetal bovine serum
https://www.sciencedirect.com/science/article/abs/pii/S0956566323001689
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