Procedure for Replacing Fetal Bovine Serum

Usually includes the following steps:1. Choose the appropriate serum Before replacing fetal bovine serum, it is necessary to select the appropriate serum based on cell type and experimental requirements. For example, some cells have adaptability to specific brands of serum, so wh···

Why are there batch differences in the cell culture efficiency of serum, and how can batch differences be avoided as much as possible?

Because serum comes from animals and has around 1000 components, the composition and content of each batch are uncertain, and there may be differences between batches. We conduct the following three important performance tests on each batch of serum products: continuous observati···

How to perform serum inactivation correctly?

Please place the serum in a 56 ℃ water bath for 30 minutes during heat inactivation. To minimize the impact of heat inactivation on serum quality, the volume of inactivated serum should not be too large. It is best to prepare the same container with an equal volume of water (at ···

Is it necessary to perform heat inactivation on all serum?

Experiments have shown that heat quenching and blood activating clearing are not necessary for most cells. The serum treated in this way only has a slight promotion of cell growth, or has no effect at all, and is often reduced in cell growth rate due to the high temperature treat···

Why do we need heat inactivated serum?

Heating can inactivate the complement system. The activated complement participates in cell lysis events, stimulates smooth muscle contraction, releases histamine from cells and platelets, and activates lymphocytes and macrophages. It is recommended to use heat inactivated serum ···

How to avoid the formation of sediment?

We suggest that you pay attention to the following operations when using serum: ① When thawing serum, please follow the recommended step-by-step thawing method (-15 ℃ to 4 ℃ to room temperature), if the temperature during serum thawing. The change is too large (such as -15 ℃ ···

Why does fetal bovine serum stored in the refrigerator precipitate?

When stored at 2-8 ℃, various proteins and lipoproteins in fetal bovine serum (such as cold agglutinin, fibrinogen, hyacinin, etc.) may aggregate and form precipitates or visible turbidity. The production of these precipitates will not affect the quality of serum. It is recommen···

What should be done if flocculent substances are found after serum thawing?

Depending on the cells being cultured, if introducing cells, maintain the original culture conditions of the cells, including the type of culture medium used. If it is to create new primary cells, it is best to use a simple formula as the basis and add specific ingredients based ···

How to thaw serum without compromising product quality?

Depending on the cells being cultured, if introducing cells, maintain the original culture conditions of the cells, including the type of culture medium used. If it is to create new primary cells, it is best to use a simple formula as the basis and add specific ingredients based ···

The best way to preserve serum

Depending on the cells being cultured, if introducing cells, maintain the original culture conditions of the cells, including the type of culture medium used. If it is to create new primary cells, it is best to use a simple formula as the basis and add specific ingredients based ···