Please place the serum in a 56 ℃ water bath for 30 minutes during heat inactivation. To minimize the impact of heat inactivation on serum quality, the volume of inactivated serum should not be too large. It is best to prepare the same container with an equal volume of water (at the same temperature as the serum). When inactivating, place the container containing both serum and water in a 56 ℃ water bath, and place a thermometer in the container containing water. During the heating process, gently rotate and mix the serum. When the thermometer shows around 56 ℃, start timing.
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