Experiments have shown that heat quenching and blood activating clearing are not necessary for most cells. The serum treated in this way only has a slight promotion of cell growth, or has no effect at all, and is often reduced in cell growth rate due to the high temperature treatment affecting the quality of the serum. After heat treatment, the sediment in the serum will significantly increase. When observed under an inverted microscope, these sediments, like "small black dots," often lead researchers to mistakenly believe that the serum has been contaminated. However, placing the serum in a 37 ℃ environment will further increase these sediments, leading researchers to mistakenly believe that it is the division and expansion of microorganisms. Therefore, we suggest that if not necessary, you do not need to undergo heat inactivation treatment. In this way, not only does it save you valuable time, but it also ensures the quality of the serum!
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