Spinal cord injury can lead to nerve tissue damage. However, the limited regenerative ability of axons after spinal cord injury in adult mammals leads to persistent neurological dysfunction. Therefore, exploring pathways to promote axonal regeneration after spinal cord injury is of great significance. Professor Sun Yi from Xiangya Hospital of Central South University used single-cell RNA sequencing to discover a unique subpopulation of bone marrow mesenchymal stem cells, which has the ability to promote axonal regeneration. Subsequently, the CD271+CD56+BMSCs subgroup was isolated by flow cytometry, and the exosomes derived from this subgroup (CD271+CD46+BMSC Exos) were extracted and incorporated into the hydrogel to create a sustained release system. The aim is to investigate the therapeutic effects of CD271+CD56+BMSC Exos and elucidate the potential mechanisms for promoting axonal regeneration and neural function recovery. Research has shown that CD271+CD56+BMSC exosomes have similar physical and chemical properties to traditional exosomes. Importantly, in the SCI model, in situ implantation of CD271+CD56+BMSC Exos hydrogel resulted in increased expression of NF and synaptophysin, which were associated with axonal regeneration and synapse formation, respectively. This intervention also helped to improve neurological recovery. In conclusion, the implantation of CD271+CD56+BMSC Exos hydrogel provides a promising and effective treatment for SCI.
The study was published on December 1, 2023 in the international journal Theranos, titled Exosomes derived from CD271+CD56+bone marrow mesenchymal stem cell subpopulation identified by single cell RNA sequencing promoting axon regeneration after spinal cord injury.
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Figure 1. The study was published in the international journal Theranos
Figure 2. Images in the article
Figure 3. Cell culture in this study was conducted using Opsey fetal bovine serum
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