Whole blood: All blood that has been anticoagulated.
Plasma: refers to a liquid obtained by centrifuging and precipitating whole blood that has been anticoagulated and has no cellular components. It contains fibrinogen (which can be converted into fibrin and has a coagulation effect). If calcium ions are added to plasma, it will undergo re coagulation, so plasma does not contain free calcium ions.
Serum: refers to the liquid released by the coagulation of blood clots in vitro, which no longer contains fibrinogen but contains free calcium ions. If calcium ions are added to it, the serum will not coagulate again.