1. The components of bovine serum
Bovine serum is a commonly used cell culture additive, mainly derived from the blood of fetal, newborn, or adult cattle. It contains various components that can provide necessary nutrients for cells and promote cell growth and proliferation.
Table 1: Composition of bovine serum
Classification | Components |
Protein class | Serum proteins (albumin, globulin), transporters (transferrin, etc.), adhesion/expansion factors, enzymes |
Carbohydrates | Glucose, galactose, etc. |
Fatty acids+lipids | Apolipoproteins, cholesterol, phospholipids, ethanolamine. |
growth factor | EGF, FGF, ECGF/PDGF, interleukin, TGFS. |
hormone | Insulin, thyroid hormones, growth hormone corticosteroids, etc |
Non protein nitrogen | Amino acids, purines, pyrimidines |
trace element | Selenium, zinc, copper, manganese, molybdenum,etc |
There are over a thousand known components of bovine serum, and the following is a list of the main components:
Figure 1: Concentration of bovine serum components:
2. Differences in bovine serum
Table 2: Comparison of Differences between Bovine Serum
Newborn bovine serum | Calf serum | ||
Blood collection time | Serum obtained through fetal bovine cardiac puncture at 3-8 months of pregnancy in cows | Serum obtained from venous blood collection and isolation of healthy newborn cows that have not been breastfed within 14 hours of birth | Serum obtained from venous blood collection and isolation of healthy live cattle within 10 months of birth |
Components and proportions | The components and proportions of growth promoting factors, adhesion promoting factors, hormones, and other active substances contained in fetal bovine serum vary. Fetal bovine serum, in particular, contains growth factors essential for embryonic development. Fetal bovine serum>newborn bovine serum>calf serum. |
Figure 2: Comparison of some detection items between fetal bovine serum and newborn bovine serum
Figure 3: Comparison of specific points between fetal bovine serum and newborn bovine serum
Figure 4: Comparison of Cell Growth Status under Different Serum and Culture Times
Adult bovine serum: 1A (12h), 2A (24h), 3A (48h); Newborn bovine serum: 1B (12h), 2B (24h), 3B (48h); Fetal bovine serum: 1C (12h), 2C (24h), 3C (48h)
Figure 5: Comparison of different serum cell growth curves
Note: The vertical axis represents cell proliferation; The horizontal axis represents the measurement time; Blue represents fetal bovine serum culture medium; Green represents newborn bovine serum culture medium; Red represents bovine serum culture medium. Number of cells per well in the experiment