Fetal bovine serum is obtained by cardiac puncture blood collection during the slaughter of pregnant cows.
However, the definition of fetal bovine serum varies among different countries' pharmacopoeias. The United States Pharmacopeia stipulates that fetal bovine serum is obtained from the fetuses of healthy prenatal cows, which are considered suitable for human consumption after examination by licensed veterinarians before and after slaughter.
The European Pharmacopoeia stipulates that fetal bovine serum is taken from unborn cattle.
The 2020 edition of the Pharmacopoeia of the People's Republic of China defines fetal bovine serum as: whole blood of fetal cattle aged 230-240 days collected from the heart, separated from the serum, filtered out of bacteria, and mainly used for cell culture.
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