Drug screening is the process of selecting compounds with high activity towards a specific target from a large number of compounds or new compounds through standardized experimental methods. It is a necessary step in clinical drug development.
Serum plays a huge role in drug screening. By adding drugs to a culture medium containing serum, the effects of drugs on biological processes such as cell growth, proliferation, and differentiation can be observed, thereby evaluating the efficacy and safety of drugs. In addition, serum can also be used to construct drug tolerant cell models, providing strong support for drug development.
However, there are also some precautions when using serum, such as selecting suitable serum based on experimental design and cell type, and using the same batch of serum as much as possible to complete the entire experiment, in order to reduce the impact of inter batch differences on experimental results. Serum should be stored at negative 20 degrees or negative 80 degrees and avoid repeated freezing and thawing. During the operation, sterile techniques should be used to avoid microbial contamination of the serum. Serum contains various growth factors and nutrients, which may affect the growth status of cells and the cytotoxicity of drugs. The most important thing is to pay attention to the compatibility between serum and drugs. In drug screening, it is necessary to consider the possible interaction between serum components and the tested drug, which may affect the activity of the drug.
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