Cell
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细胞资源

Human small cell lung cancer cells (SHP-77)

Introduction

SHP-77 cells were established in 1977 by Edwin R. Fisher and John D. Paulson from an unencapsulated primary lung tumor located at the top of the left upper lobe in a 54 year old white male.

Goods No.:JYK-CT-0197
Format:1x10E6cells/T25 bottle
Price:discuss personally
Quantity:1
Culture System:1640+10% FBS+1% P/S+1% glutamine+1% sodium pyruvate
Update time:2024-10-23
Visits:192

1、 Basic cellular information

【Cell Name】Human small cell lung cancer cells

【Cell alias】SHP-77

【Species of origin】 Humans

【Tissue source】 Lung; small cell lung cancer

【Cell morphology】Suspended and loosely adherent

【Growth feature】 Suspended growth

【 Culture System】 1640+10% FBS+1% P/S+1% glutamine+1% sodium pyruvate

【Proportion of passage】 1:2-1:3, change the medium every 2-3 days

【Condition of culture 】 Gas phase: 95% air+5% carbon dioxide, temperature: 37 ℃

【Freehold conditions】Freezing solution: 55% basic medium+40% FBS+5% DMSO; Storage conditions: liquid nitrogen

【Safety】It is recommended to operate within the second level biosafety platform and take personal protective measures.

【Purpose】For scientific research purposes only

2、 Recovery and passage methods

1. Cell line recovery (cryotube)

(1) Quickly and completely melt the cryovial in a 37 ℃ water bath, and gently shake it to promote melting. Rapid and complete melting can improve the recovery effect of cells.

(2) Before opening the cryovial, wipe the outer wall of the cell cryovial with 75% alcohol.

(3) Centrifuge the completely melted cells directly, or transfer them to a sterile 15ml or other suitable sterile centrifuge tube, centrifuge at 400g for 5 minutes, aspirate the supernatant, be careful not to aspirate the cell sediment, then resuspend in fresh complete culture medium and transfer to a culture vessel, mix well, and incubate in a CO2 incubator at 37 ℃.

(4) The next day, depending on the adhesion or growth status, replace the culture medium.

Attention: If the received cryovials cannot be resuscitated in a timely manner, please store them temporarily at -80 ℃ or liquid nitrogen and resuscitate them for cultivation as soon as possible.

2. Cell line revival (T25 bottle)

(1) Wipe the outer wall of the T25 bottle with 75% alcohol after removing the outer packaging.

(2) Observe the cell state under an inverted microscope to see if there are any abnormalities; After no abnormalities were found, excess culture medium was removed using a pipette and incubated overnight at 37 ℃ in a CO2 incubator.

(3) The next day, depending on the adhesion or growth status, consider changing the medium or passaging, with a passaging ratio of 1:2 to 1:4.

3、 Notes

1. Each cryovial contains approximately 1 × 106 cells, with a volume of 1ml and an expected survival rate of 60-90%. It is recommended to resuspend the cells into one T25 bottle or one 6cm culture dish. If the survival rate is low after recovery, it can be digested and transferred to a 3.5cm culture dish/T12.5 bottle for better cell growth.

2. If this product is transported at room temperature and is a adherent cell filled with complete culture medium in a culture bottle, upon receipt of the cell:

(1) Take timely photos to record any leakage or damage to the bottle body.

(2) Wipe the surface of the cell culture bottle with 75% alcohol and observe the cell status under a microscope. Do not open the culture bottle cap yet, place the cells in the cell culture box and let them stand for 2-4 hours to stabilize the cell state.

(3) Carefully read the cell manual to understand cell related information.

(4) After settling, take out the cell culture bottle, examine under a microscope, take photos, and record the cell status.

3. If the cell density exceeds 85%, please proceed with passaging as soon as possible; If there are many suspended cells, please place the culture bottle in the incubator overnight to allow the suspended cells to adhere to the wall again. If the received cells are suspended in centrifuge tubes transported at room temperature, they can be directly taken out and transferred to culture dishes or bottles for cultivation. If the color of the culture medium is normal, keep the culture medium for further cultivation. When changing the culture medium for the first time, keep half of the original culture medium and add half of the fresh culture medium. This can minimize the discomfort of cell growth caused by differences in culture medium or serum, and ensure a good growth state of the cells.

4. Cell culture should be conducted in a biosafety counter and strictly follow aseptic procedures.


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