Selected literature | Extracellular vesicles from UTX knockout endothelial cells promote neural stem cell differentiation in spinal cord injury-Inner Mongolia Jin Yuan Kang Biotechnology Co., Ltd fetal bovine serum

Product Name: Fetal bovine serum

Product Code: BS-1105

Cultivate cells: vascular endothelial cells, astrocytes

Figure 1. Cell culture in this study was conducted using Oposse fetal bovine serum

Vascular endothelial cells play a crucial role in the pathophysiological processes following spinal cord injury (SCI). UTX is an important epigenetic regulator of endothelial cell phenotype. The discovery of endogenous neural stem cells (NSCs) in the spinal cord provides a new strategy for tissue repair of SCI.

In order to investigate the effect of UTX KO on neural differentiation, the team led by Professor Liu Yudong from Xiangya Hospital of Central South University obtained extracellular vesicles (EVs) from UTX KO spinal microvascular endothelial cells (SCMECs) and negative control SCMECs. Then use these EVs to regulate the differentiation trajectory of endogenous NSCs in the SCI model. In the SCI model of NSCs lineage tracking mice, a significant reduction in neurogenesis was observed after injury. It is worth noting that compared with the control group, NSCs in UTX KO SCMECs mice showed enhanced neuronal differentiation. RNA sequencing and Western blot analysis showed that L1 cell adhesion molecule (L1CAM), a gene associated with neurogenesis, was upregulated in UTX KO SCMECs and their secreted EVs. This is consistent with the observed promotion of nerve regeneration under UTX KO conditions. The administration of L1CAM rich EVs (KO EVs) from UTX KO SCMECs in mice significantly enhanced neural differentiation. Similarly, in vitro exposure of NSCs to KO EVs leads to increased activation of the Akt signaling pathway, further promoting neural differentiation. On the contrary, inhibiting Akt phosphorylation or knocking down L1CAM reversed the beneficial effect of KO EVs on neuronal differentiation of NSCs.

The research results confirm that EVs derived from UTX KO SCMECs can serve as promoting factors for neural differentiation after SCI. This study not only elucidates a new mechanism, but also opens up new perspectives for therapeutic interventions in SCI.

The study was published on February 29, 2024 in the international journal Cell Communication and Signaling, titled Extracellular vesicles from UTX knock out endothelial cells boost neural stem cell diversion in spinal cord injury.

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